AAV Capsid Database
VERIFIED ## Connections
gene-therapy aav vector-design
What It Does
- Catalog of natural and engineered AAV capsids
- Tropism profiles (which tissues each serotype infects)
- Packaging capacity per serotype
- Neutralizing antibody cross-reactivity data
- Engineering strategies (directed evolution, rational design)
How to Use
### Web
- https://aavdb.org (if available) or search literature
- Also see: AAV Atlas project
### Key Serotypes for Inner Ear
Verified Status
VERIFIED — compiled inner ear AAV serotype data from literature:
- Anc80L65: IHC + OHC tropism, 5-10% seroprevalence, BEST candidate for STRC (Landegger 2017)
- AAV1: IHC + OHC, ~30% seroprevalence, used in OTOF trials
- AAV9: IHC + some OHC, ~40% seroprevalence
- AAV-ie: IHC + OHC engineered, seroprevalence unknown (Tan 2019)
- STRC CDS 5,325bp exceeds AAV limit (~4.7kb) → mini-STRC (700-1775) = 3,228bp FITS all serotypes
STRC Research Usage
- STRC AAV Vector Design — serotype selection for STRC delivery
- STRC Gene Therapy Research — vector design context
- STRC is 5,427 bp CDS — exceeds AAV packaging limit (~4.7kb) → needs mini-STRC or dual-vector approach
Results (April 2026)
- Capacity analysis DONE: mini-STRC (700-1775) = 3,228bp → FITS all AAV serotypes (limit ~4.7kb). Full STRC 5,325bp does NOT fit.
- OHC serotypes DONE: Anc80L65 and AAV-ie have best OHC tropism. AAV1 also transduces OHC.
- Immune profile DONE: Anc80L65 ~5-10% seroprevalence (lowest). AAV1 ~30%, AAV9 ~40%. Misha age 4 = low seroprevalence window.
- Next: delivery route comparison (round window vs canalostomy) — requires clinical literature review
Results (April 2026)
- Capacity analysis DONE: mini-STRC (700-1775) = 3,228bp → FITS all AAV serotypes (limit ~4.7kb). Full STRC 5,325bp does NOT fit.
- OHC serotypes DONE: Anc80L65 and AAV-ie have best OHC tropism. AAV1 also transduces OHC.
- Immune profile DONE: Anc80L65 ~5-10% seroprevalence (lowest). AAV1 ~30%, AAV9 ~40%. Misha age 4 = low seroprevalence window.
- Next: delivery route comparison (round window vs canalostomy) — requires clinical literature review