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Clustal Omega

VERIFIED

## Connections

conservation alignment bioinformatics

What It Does

  • Multiple sequence alignment (MSA) of protein or DNA sequences
  • Supports hundreds of sequences
  • Output formats: FASTA, ClustalW, Phylip
  • Generates guide trees showing evolutionary relationships

How to Use

### Web

1. Go to https://www.ebi.ac.uk/jdispatcher/msa/clustalo

2. Paste sequences in FASTA format

3. Submit and view alignment

### API


        # Submit job
        curl -X POST "https://www.ebi.ac.uk/Tools/services/rest/clustalo/run" \
          -d "email=your@email.com&sequence=>human\nMVLSPA...\n>mouse\nMVLSPA..."

### Command Line


        # Install
        conda install -c bioconda clustalo
        
        # Run alignment
        clustalo -i strc_orthologs.fasta -o strc_aligned.fasta --outfmt=fasta

Verified Status

VERIFIED — used for STRC 9-species conservation analysis. E1659 position is 100% conserved (Glu) across all 9 mammals spanning ~80 million years of evolution.

STRC Research Usage

  • STRC Cross-Species Conservation Analysis — aligned 9 mammalian STRC sequences
  • Conservation at E1659: 100% conserved Glu across human, mouse, rat, dog, cow, pig, horse, rabbit, bat

STRC Research Usage

  • STRC Cross-Species Conservation Analysis — aligned 9 mammalian STRC sequences
  • Conservation at E1659: 100% conserved Glu across human, mouse, rat, dog, cow, pig, horse, rabbit, bat